Surmodics Stabilisers to improve assay performance
BBI offers a range of protein and non-protein based reagents designed to improve the performance of IVD assays. BBI supplies the Surmodics branded range of stabilisers to it EU based clients. StabilCoat™, StabilBlock™ and StabilGuard™ product ranges effectively preserve the conformation and activity of proteins while simultaneously blocking the surface to reduce non‐specific binding. The products can be used to stabilise antibodies, antigens or enzymes on an assortment of immunoassay components: polystyrene plates, tubes, glass, membranes, and filter paper.
The non‐specific binding of serum proteins, conjugates and other assay components to solid surfaces in immunoassay applications requires that a blocking step be incorporated into the protocol to maximize assay sensitivity. Additionally, the manufacturing of immunoassay test kits requires long-term protein stability to increase the manufacturing scale and extend shelf lives for the end user. The different formulations allow these requirements to be accomplished in a single step while easily being substituted for the blocking solution in an existing assay protocol.
StabilZyme™ conjugate/protein stabilisers protect the entire conjugate by preventing the loss of catalytic activity and maintaining the structural integrity of the protein in solution. These stabilisers allow for the storage of conjugated proteins at lower use concentrations, extended shelf life and increased signal‐to‐noise ratios for improved assay performance. Materials are provided as ready to use formulations or concentrates in specific buffered matrix designed for optimal performance.
Please contact our Customer Services Team directly to discuss your requirements on tel:+44 (0) 1495 363000 or alternatively you can submit an enquiry here.
IMMUNOASSAY STABILIZERS / BLOCKERS
| Product | Product code | Quantity | Description |
|---|---|---|---|
| StabilCoat® Immunoassay Stabilizer | SC01-0050 SC01-1000 |
50 mL 1000 mL |
Effectively preserves the conformation and activity of dried proteins. |
| 5X StabilCoat® Immunoassay Stabilizer | SC05-0100 SC05-1000 |
100 mL 1000 mL |
• 5x concentrate • Effectively preserves the conformation and activity of dried proteins |
| StabilGuard® Immunoassay Stabilizer (BSA-Free) | SG01-0050 SG01-1000 |
50 mL 1000 mL |
Maintains the activity of antibodies and other biomolecules absorbed or immobilised to solid substrates. |
| StabilBlock® Immunoassay Stabilizer | 50 mL 1000 mL |
Minimizes non-specific binding to lower detection limits and maximizes signal-to-noise ratios of immunoassays. | |
| StabilBlock® Immunoassay Stabilizer-CMIT/MIT | ST01-CF01-0050 ST01-CF01-1000 |
50 mL 1000 mL |
Newly developed formulation containing a mixture of 5-Chloro-2-Methyl-3(2H)-isothiazolone and 2-Methyl-3(2H)-isothiazolone as preservative. |
STABILIZERS FOR MICROSPHERE-BASED ASSAYS AND MICROARRAY SLIDES
| Product | Product code | Quantity | Description |
|---|---|---|---|
| StabilCoat® Plus Microarray Stabilizer | SC02-0050 SC02-1000 |
50 mL 1000 mL |
Improves the stability of proteins bound to microspheres stored in solution, and array proteins stored in the dried state. |
| StabilGuard® Choice Microarray Stabilizer (BSA-Free) | SG02-0050 SG02-1000 |
50 mL 1000 mL |
Improves the stability of proteins bound to microspheres stored in solution, and array proteins stored in the dried state. |
PROTEIN AND CONJUGATE STABILIZERS
| Product | Product code | Quantity | Description |
|---|---|---|---|
| StabilZyme® AP Conjugate Stabilizer | SA01-0050 SA01-1000 |
50 mL 1000 mL |
Developed specifically for Alkaline Phosphatase (AP) conjugates in solution. It prevents the loss of catalytic activity and maintains stability of proteins in solution. |
| StabilZyme® AP Conjugate Stabilizer-CMIT/MIT | 50 mL 1000 mL 2000 mL |
This alternative formulation contains a mixture of 5-Chloro-2-Methyl-3(2H)-isothiazolone and 2-Methyl-3(2H)-isothiazolone as preservative. | |
| StabilZyme® Protein-Free AP Stabilizer | SAPF-0050 SAPF-1000 |
50 mL 1000 mL |
Optimised to prevent the loss of Alkaline Phosphatase (AP) activity while maintaining the conformational structure and binding efficacy of the conjugated antibody. |
| StabilZyme® Protein-Free AP Stabilizer-CMIT/MIT | 50 mL 1000 mL |
This alternative formulation contains a mixture of 5-Chloro-2-Methyl-3(2H)-isothiazolone and 2-Methyl-3(2H)-isothiazolone as preservative. | |
| StabilZyme® HRP Conjugate Stabilizer | 50 mL 1000 mL |
Developed specifically for Horseradish Peroxidase (HRP) conjugates in solution and is readily incorporated into virtually any assay production protocol. | |
| StabilZyme® HRP Conjugate Stabilizer-CMIT/MIT | 50 mL 1000 mL |
This alternative formulation contains a mixture of 5-Chloro-2-Methyl-3(2H)-isothiazolone and 2-Methyl-3(2H)-isothiazolone as preservative. | |
| StabilZyme SELECT® Stabilizer | 50 mL 1000 mL |
Stabilises biologically active components in solution and protects the activity of proteins commonly used in immunoassays, such as antisera controls and HRP conjugates. | |
| StabilZyme SELECT® Stabilizer-CMIT/MIT | 50 mL 1000 mL |
This alternative formulation contains a mixture of 5-Chloro-2-Methyl-3(2H)-isothiazolone and 2-Methyl-3(2H)-isothiazolone as preservative. | |
| StabilZyme® NOBLE Stabilizer (BSA-Free) | 50 mL 1000 mL |
Recommended for biologically active components in solution, including enzyme conjugates, conjugated proteins, antibody-coated particles, and unmodified proteins. | |
| StabilZyme® NOBLE Stabilizer (BSA-Free) CMIT/MIT | SZ04-CF02-0050 SZ04-CF02-1000 |
50 mL 1000 mL |
This alternative formulation contains a mixture of 5-Chloro-2-Methyl-3(2H)-isothiazolone and 2-Methyl-3(2H)-isothiazolone as preservative. |
| StabilZyme® Protein-Free Stabilizer | 50 mL 1000 mL |
Designed for conjugated proteins, antibody-coated particles, and unmodified proteins in solution. It delivers top performance in a protein-free formulation. | |
| StabilZyme® Protein-Free Stabilizer-CMIT/MIT |
50 mL 1000 mL |
This alternative formulation contains a mixture of 5-Chloro-2-Methyl-3(2H)-isothiazolone and 2-Methyl-3(2H)-isothiazolone as preservative. |
STABILBLOT™ BLOCKERS
| Product | Product code | Quantity | Description |
|---|---|---|---|
| StabilBlot™ Protein-Free Blocker | SB01-0125-01 SB01-1000-01 |
125 mL 1000 mL |
• Ready to use • The absence of protein in the formulation provides a synthetic alternative that eliminates cross-reactivity and increases sensitivity |
| StabilBlot™ Milk Blocker (10X) | SB04-0125-01 SB04-1000-01 |
125 mL 1000 mL |
• 10x concentrate • For the strongest blocking, the milk formulation is recommended |
| StabilBlot™ Casein Blocker (5X, TBS) | SB05-0125-01 SB05-1000-01 |
125 mL 1000 mL |
• 5x concentrate • For strong blocking of background, a casein TBS and a casein PBS formulation are available |
ASSAY DILUENTS
| Product | Product code | Quantity | Description |
|---|---|---|---|
| Surmodics Assay Diluent (Protein-Free) | SM01-0050 SM01-1000 |
50 mL 1000 mL |
Surmodics Assay Diluent reduces matrix interference in samples including heterophilic antibodies such as HAMA (human anti-mouse antibody) and RF (rheumatoid factor). |
| Surmodics Assay Diluent (Protein-Free)-CMIT/MIT | SM01-CF02-0050 SM01-CF02-1000 |
50 mL 1000 mL |
New formulation of Surmodics Assay contains a mixture of 5-Chloro-2-Methyl-3(2H)-isothiazolone and 2-Methyl-3(2H)-isothiazolone as preservative. |
| Surmodics MatrixGuard™ Diluent | SM02-0050 SM02-1000 |
50 mL 1000 mL |
Provides unsurpassed blocking whereby matrix interferences are effectively blocked while the intended assay signal is maintained. |
