Product Details
MAB40 is part of BBI's anti-Biotin Gold Conjugate family which include anti-Biotin antibodies or streptavidin conjugated to BBI gold nanoparticles for use in the detection of biotinylated markers. Monoclonal anti-Biotin (MAB) conjugated to 40nm colloidal gold nanoparticles can be produced in large volumes of up to 24L OD10 per batch. The antibody is a high quality monoclonal antibody produced in mouse ascites and protein G purified to give a purity of greater than 90%. It is passively coupled to citrate capped gold nanoparticles and blocked with bovine serum albumin (BSA) For customers moving away from ELISA to lateral flow, biotinylated antibodies will generate gold labelled antibody conjugates in one simple step. Generally, biotin attaches to the Fc part of the antibody, leaving the variable part free to react with the antigen and form a fully active conjugate. Suitable for use in lateral flow devices. It can utilised in a range of matrices including whole blood, serum, urine, saliva and milk and can be dried down onto glass fibre/lyophilised easily without affecting its activity. The conjugate is suspended in a low molarity buffer 2mM disodium tetraborate buffer, pH7.2, containing 0.095% Sodium Azide as preservative. This buffer will maintain the activity of the antibody attached to the surface of the gold colloid, without causing particle aggregation, and can easily be substituted for a customers buffer of choice for their assay. This product can be purchased online in 5ml or 100mL volumes at OD10. For larger volumes, please discuss your requirements with our sales team. Customised concentrations of conjugates to an OD other than OD10 are also available by request.
