ALPI2F-Alkaline phosphatase

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ALPI2F-Alkaline phosphatase

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#SKU ALPI2F

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Product Details

Alkaline Phosphatase (ALP) is commonly used as a label in immunoassays such as ELISA, and in blotting and histochemistry. Once conjugated to antibodies, antigens, or streptavidin, its low backgrounds and linear reaction rate enable increased sensitivity over extended incubation times. It can be used with a variety of substrates producing precipitated or soluble chromogens, or with chemiluminescent and fluorogenic substrates for enhanced sensitivity. BBI Solutions (BBI) is the world’s leading producer of bovine ALP and has supplied the diagnostics industry with the widest range and highest specific activity material for over 25 years. Our raw materials selection, harvesting protocol and production procedures have been optimised to ensure that we achieve the highest possible specific activity and batch-to-batch consistency. We have also developed a series of preparations to provide a range of specific activities to suit a wide range of applications. Our many commercial partners have come to rely on ALP from BBI for proven performance in their immunoassay systems.

ALPI2F

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Attributes

Description

  • Abbreviations
  • ALP, Alk. Phos
  • Associated Activity
  • Protein Concentration: 0.35 – 0.75 mg/mg (Determined by Biuret procedure)
  • Lead Time
  • 12 weeks
  • Storage
  • Store desiccated at -15°C or below. Allow to come
  • Maximum Assigned Shelf Life
  • 2 years
  • Source
  • Bovine Intestinal mucosa
  • Activity
  • > 5 Glycine U/mg
  • EC Number
  • 3.1.3.1
  • EINECS
  • 232-631-4
  • Solubility
  • Dissolves readily at 5mg/ml in 0.025M glycine/NaOH pH 9.6 containing 1mM Magnesium chloride, 0.1mM Zinc chloride and 10% glycerol to give a clear solution.
  • Systematic Name
  • Orthophosphoric-monoester phosphohydrolase (alkaline optimum).

Technical Documents

Product Specification (104.03KB)

Analytical Procedures (140.23KB)

Product Information (513.63KB)

Method Analytical Procedure (131.28KB)

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