High purity enzymes for glycobiology research, diagnostic devices and production of low molecular weight heparin.
After acquiring IBEX, BBI Solutions is now the largest manufacturer of heparinases in the world.
IBEX has developed a proprietary expression system for the production of its enzymes in Flavobacterium heparinum. This system allows for the production of each glycosaminoglycan enzyme such that it performs exactly like enzymes produced from the native strain of Flavobacterium heparinum (Pedobacter heparinus) but minimizes the cross-contamination of other GAG enzymes which can occur when enzymes are produced in the native strain.
- Recombinant enzymes fold exactly like native-produced enzymes
- Recombinant enzymes are glycosylated like the native-produced enzymes
- Recombinant enzymes do not contain preservatives or external proteins such as serum albumin (BSA)
- Recombinant enzymes are available in liquid or lyophilized form
When combined with our well-defined proprietary purification process our enzymes are of the highest purity.
Due to its high purity and reaction time, our Heparinase I is the only heparinase used in FDA and EU-approved hemostasis-measuring Point of Care devices.
Liquid Enzymes (shipped on dry ice)
| Product | Form | Part No. | IU per Vial |
|---|---|---|---|
| Heparinase I | Liquid (Frozen) | 50-010-001 | 0.10 |
| Heparinase I | Liquid (Frozen) | 50-010 | 0.50 |
| Heparinase II | Liquid (Frozen) | 50-011-001 | 0.10 |
| Heparinase II | Liquid (Frozen) | 50-011 | 0.50 |
| Heparinase III | Liquid (Frozen) | 50-012-001 | 0.10 |
| Heparinase III | Liquid (Frozen) | 50-012 | 0.50 |
Lyophilized Enzymes
| Product | Form | Part No. | IU per Vial |
|---|---|---|---|
| Heparinase I | Lyophilized | 60-010 | 0.50 |
| Heparinase I | Lyophilized | 60-012 | 2.00 |
| Heparinase I | Lyophilized | 60-014 | 10.00 |
| Heparinase I | Lyophilized | 60-017 | 100.00 |
| Heparinase II | Lyophilized | 60-018 | 0.50 |
| Heparinase III | Lyophilized | 60-020 | 0.50 |
Please note that the the above definition of units differs from the Sigma definition.
IBEX enzymes are sold in “International Units”. For heparinase this means that one international unit (IU) is defined as the amount of enzyme that will liberate 1.0 µmole unsaturated oligosaccharides from heparin or heparan sulfate per minute (at 30 °C and pH 7.5)
Sigma Units are defined as follows:
One unit (Sigma unit) will form 0.1 μmole of unsaturated uronic acid per hour at pH 7.5 at 25 °C
Therefore one unit (IU) is equivalent to approximately 600 Sigma units.