Holo Transferrin


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+ Over 98% purity

+ Batch-to-batch consistency

+ Research-grade prices

Mammalian cell culture in a serum free medium requires an iron transporter such as transferrin. Human transferrin is a 77 kDa beta-glycoprotein with a range of phenotypes based upon the number of sialic acid residues. Each molecule of transferrin specifically binds two Fe3+ molecules.

The iron content can be adjusted to give near 100% saturation to yield holo-transferrin (Iron 1200-1600 ug/gm) or depleted to give near zero iron bound to yield apo-transferrin (Iron <50ug/gm protein) or the intermediate- sidero-transferrin (Iron 300-1000ug/gm protein).

The secondary role for transferrin in culture media is to bind endogenous metal iron which may cause cell toxicity.

More Information
Abbreviation Holo Tnf
Applications Mammalian Cell Culture
CAS Number 11096-37-0
Concentration 1200 - 1700ppm
Delivery Time 1 week
Disease Area Plasma Proteins
Health and Safety

Derived from human plasma with full traceability to the donor. Units and pools have been screened and found negative for the recommended list of infectious agents.

Keywords transferrin, holo transferrin, iron saturated transferrin
Presentation Matrix Single homogenous batch, heat treated at 62oC å± 2oC for 10 hours and lyophilised from approximately 0.020M NH4HCO3 solution. May contain traces of buffer salts.
Product Category Cell Culture Reagents
Purity/Grade > 98% pure
Recommended Use

Research and manufacture

Source Human serum
Specification 1200 - 1700ppm iron content
Storage Store at 2 -8°C
Tested Applications cell proliferation of K562, HUT-78, RAJI and JURKAT cells.
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