Why choose BBI?
+ Over 98% purity
+ Batch-to-batch consistency
+ Research-grade prices
Mammalian cell culture in a serum free medium requires an iron transporter such as transferrin. Human transferrin is a 77 kDa beta-glycoprotein with a range of phenotypes based upon the number of sialic acid residues. Each molecule of transferrin specifically binds two Fe3+ molecules.
The iron content can be adjusted to give near 100% saturation to yield holo-transferrin (Iron 1200-1600 ug/gm) or depleted to give near zero iron bound to yield apo-transferrin (Iron <50ug/gm protein) or the intermediate- sidero-transferrin (Iron 300-1000ug/gm protein).
The secondary role for transferrin in culture media is to bind endogenous metal iron which may cause cell toxicity.
|Applications||Mammalian Cell Culture|
|Disease Area||Plasma Proteins|
|Keywords||human, apo transferrin, transferrin, cell culture|
|Product Category||Cell Culture Reagents|
Iron binding and transport in cell culture media
|Shipping Recommendation||Cool pack|