Alkaline Phosphatase (ALP)
ALP is the other enzyme of choice for the majority of antibody conjugations. It is often used as an alternative to HRP when a more linear reaction rate is required over a wide range of values. The source of ALP with the highest specific activity, and therefore of most use in assays, is calf intestine.
BBI’s ALPI12G is our highest specific activity product and bestselling line. Its performance has been proven in assays manufactured by some of the largest immunoassay and IVD companies.
Why Use BBI Alkaline Phosphatase?
we achieve up to 2300 glycine U/mg protein and 7000 DEA U/mg so you can be confident in the performance of your assay
our product experiences <10% activity lost over 48 months ensuring reliable and consistent performance of your assay
BBI offer ALP in a range of specific activities, including blocking grades, which allows you to pick the best product for your specific application
large batch sizes allow us to fulfil multiple orders from a single batch reducing validation costs
IN DETAIL
Alkaline Phosphatase (ALP)
Alkaline Phosphatase (ALP) is commonly used as a label in immunoassays such as ELISA, and in blotting and histochemistry. Once conjugated to antibodies, antigens, or streptavidin, its low backgrounds and linear reaction rate enable increased sensitivity over extended incubation times.
It can be used with a variety of substrates producing precipitated or soluble chromogens, or with chemiluminescent and fluorogenic substrates for enhanced sensitivity. BBI is the world’s leading producer of bovine ALP and has supplied the diagnostics industry with the widest range and highest specific activity material for over 25 years.
Our raw materials selection, harvesting protocol and production procedures have been optimised to ensure that we achieve the highest possible specific activity and batch-to-batch consistency. We have also developed a series of preparations to provide a range of specific activities to suit a wide range of applications. Our many commercial partners have come to rely on ALP from BBI for proven performance in their immunoassay systems
FAQs
What is Alkaline Phosphatase made from?
BBI Solutions’ Alkaline Phosphatase (ALP) is extracted from bovine intestine then purified using multiple precipitation, fractionation, chromatography and filtration steps.
Where can ALP be used?
ALP is commonly used as a label in immunoassays such as ELISA, and in blotting and histochemistry. Once conjugated to antibodies, antigens, or streptavidin, its low backgrounds and linear reaction rate enables increased sensitivity over extended incubation times. It can be used with a variety of substrates producing precipitated or soluble chromogens, or with chemiluminescent and fluorogenic substrates for enhanced sensitivity.
How is ALP supplied?
Our high activity conjugation grade products are supplied as glycerol solutions.
Which is the most popular ALP grade to use as a marker enzyme in ELISA?
BBI grade ALPI12G, which has the highest specific activity currently available.